Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.

Abstract

BioTechniquesVol. 34, No. 4 BenchmarkOpen AccessStoichiometry of Site-Specific Protein Phosphorylation Estimated with Phosphopeptide-Specific AntibodiesCristinel P. Mîinea & Gustav E. LienhardCristinel P. MîineaDartmouth Medical School, Hanover, NH, USASearch for more papers by this author & Gustav E. Lienhard*Address correspondence to: Dr. Gustav E. Lienhard, Department of Biochemistry, Vail Building, Dartmouth Medical School, Hanover, NH 03755, USA. e-mail: E-mail Address: gustav.e.lienhard@dartmouth.eduDartmouth Medical School, Hanover, NH, USASearch for more papers by this authorPublished Online:24 Sep 2018https://doi.org/10.2144/03344pt01AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByCompositional Analysis of Asymmetric and Symmetric Dimethylated H3R2 Using Liquid Chromatography–Tandem Mass Spectrometry-Based Targeted Proteomics8 August 2016 | Analytical Chemistry, Vol. 88, No. 17An ID2-dependent mechanism for VHL inactivation in cancer6 January 2016 | Nature, Vol. 529, No. 7585A Protein Kinase C Phosphorylation Motif in GLUT1 Affects Glucose Transport and is Mutated in GLUT1 Deficiency SyndromeMolecular Cell, Vol. 58, No. 5Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics27 March 2015 | Nature Communications, Vol. 6, No. 1Simultaneous Quantification of Protein Phosphorylation Sites using Liquid Chromatography–Tandem Mass Spectrometry-Based Targeted Proteomics: A Linear Algebra Approach for Isobaric Phosphopeptides17 November 2014 | Journal of Proteome Research, Vol. 13, No. 12Dysregulation of Glycogen Synthase COOH- and NH2-Terminal Phosphorylation by Insulin in Obesity and Type 2 Diabetes MellitusThe Journal of Clinical Endocrinology & Metabolism, Vol. 94, No. 11Critical role of novel Thr-219 autophosphorylation for the cellular function of PKCθ in T lymphocytes27 October 2005 | The EMBO Journal, Vol. 24, No. 22The α2–5′AMP-Activated Protein Kinase Is a Site 2 Glycogen Synthase Kinase in Skeletal Muscle and Is Responsive to Glucose LoadingDiabetes, Vol. 53, No. 12Current Awareness on Comparative and Functional GenomicsComparative and Functional Genomics, Vol. 4, No. 5 Vol. 34, No. 4 Follow us on social media for the latest updates Metrics Downloaded 150 times History Published online 24 September 2018 Published in print April 2003 Information© 2018 Author(s)PDF download