Stimulatory Effect of Purine and Pyrimidine Bases and their Role in the Mediation of Auxin Action through the Regulation of Carbohydrate Metabolism during Adventitious Root Formation in Hypocotyl Cuttings of Phaseolus mungo

Abstract

Summary Exogenous application of purine and pyrimidine bases in combination with auxin and sucrose hastened and enhanced the production of roots in hypocotyl cuttings of Phaseolus mungo. The changes in the biochemical drifts in dry matter, carbohydrates, amylase and invertase at different stages of root formation show that: Dry matter content of cuttings increased in sucrose as well as IAA + Suc without or with base after 12hr but remained low in water, IAA and base alone till 48hr synchronising with an increase in carbohydrate content at 18hr in IAA + Suc with or without a base as well as in sucrose alone. The amylase activity increased in all the cultures during 12-24 hr while the invertase activity increased during 6-12 hr to decline subsequently to increase again at 24 hr. The significance of these enzymes in relation to nitrogenous bases on the differentiation of cainbial derivatives into roots has been discussed.