Stimulation of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor evokes mixed responses in nociceptive fibers of mouse glabrous skin

Abstract

This study examined the contribution of AMPA receptors to peripheral nociception using teased plantar nerve attached to glabrous skin of male C57B/6 mice. Identified receptive fields were isolated using a plastic well. After measuring mechanical (MECH), heat and cold responses, serial 10X dilutions of AMPA ranging from 1 pM to 1 mM were pipetted into the well. Firing activity was collected over 2 min for each dilution. After a 2 min wash-out, MECH, heat, and cold responses were re-measured. A 50% increase or decrease in threshold or firing rate was considered significant.