Abstract
Still another activity by the highly promiscuous enzyme CYP3A4: 25-hydroxylation of cholesterol
Highlights
25-Hydroxycholesterol (25OHC) is the most widely used oxysterol in connection with biochemical experiments designed to evaluate sensitivity of various biological systems against sterols
It has previously been shown that CYP3A4 is able to catalyze 25-hydroxylation of vitamin D, 1a-hydroxyvitamin D, and 5b-cholestane-3a,7a, 12a-triol [9, 10]
This study was not designed for studies on conversion of endogenous cholesterol into 25OHC, and a small conversion of added exogenous radioactive cholesterol that had been diluted with endogenous cholesterol may have been difficult to detect
Summary
25-Hydroxycholesterol (25OHC) is the most widely used oxysterol in connection with biochemical experiments designed to evaluate sensitivity of various biological systems against sterols. According to the investigation by Honda et al presented in this issue of the Journal of Lipid Research [7], 25OHC in the human circulation may have an additional origin. Cells overexpressing human recombinant CYP3A4 were shown to have a significant 25-hydroxylase activity toward cholesterol.
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