Abstract
Substances that can be used as photosensitizers for cardiac tissue are very helpful in modeling various excitation patterns in a cardiac tissue culture and may have prospective use in the temporary and permanent ablation of unwanted excitation sources in the heart.The aim of the present work is to study the effect of stilbene derivative c-TAB (2- {4- [(E) -2- (4-ethoxyphenyl) vinyl] phenoxy} ethyl) trimethylammonium bromide) on the cardiomyocyte layers and voltage-gated ion channels in cardiac cells. C-TAB is a structural analog to AzoTAB, reported previously as a photoswitch for cardiac and neural cells, in which the azobenzene moiety is replaced by a stilbene grouping. Such a replacement makes c-TAB less toxic to living cells. c-TAB has been shown to successfully inhibit excitation in cardiac cells in both trans- and cis- forms. The excitation inhibition of cardiac cells under c-TAB is reversible and can be overturned easily by washing out the c-TAB; however, not by light illumination. The irradiation of cardiac cells with near-UV, when the trans- form of c-TAB is applied, changes reversible inhibition to a permanent one that cannot be overturned by a washout.
Highlights
A method, which allows photocontrol of the excitation waves in cardiac tissue, potentially, has two major fields of applications
We reported a method of photocontrol of cardiomyocyte layers with the aid of the azobenzene derivative azobenzenetrimethylammonium bromide (AzoTAB) [1,2]
The present work is devoted to the study of the effect of stilbene derivative stilbene trimethylammonium bromide (c-TAB) (2- {4- [(E) -2- (4-ethoxyphenyl) vinyl] phenoxy} ethyl) trimethylammonium bromide) [11] on the cardiomyocyte layers and voltage-gated ion channels in cardiac cells. c-TAB is a structural analog to AzoTAB in which the azobenzene moiety is replaced by a stilbene grouping (Figure 1A)
Summary
A method, which allows photocontrol of the excitation waves in cardiac tissue, potentially, has two major fields of applications. Controlling excitability of cardiac cells in a tissue culture in a patterned way provides a very convenient tool when studying excitation propagation in various conduction pathways and varying conditions. It may have a prospective clinical use, for example, as a temporary and less damaging substitute for the ablation procedures, removing unwanted conduction circuits and excitation sources in a heart. By projecting computer-generated patterns on the AzoTAB-sensitized cardiomyocyte layers, it was possible to obtain temporary pathways for propagating excitation waves as well as for studying the behavior of cardiac reentry waves [3,4] in the tissue undergoing a decrease in excitability [3,4]. Among them are photoswitches that have been used by neuroscientists to modulate the activity of ion channels in neurons [6,7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
