Abstract
Nine sterols, most showing Δ 5- or Δ 5,22-unsaturation, were identified in the marine diatom Biddulphia sinensis. One sterol, cholesta-5,22 E-dien-3β-ol, comprised 70–80% of the total sterols which is the first such predominance noted in a diatom. The only Δ 7-sterol detected was cholest-7-en-3β-ol and this was a very minor component. A sterol showing unusual side-chain alkylation,23,24-dimethylcholesta-5,22 E-dien-3β-ol, was identified for the first time in a diatom. Total fatty acids exhibited a predominance of Δ 9- 16:1, 14:0, 20:5 and 16:0, typical of diatoms, although the proportions of these acids were found to vary with culture maturity. n-Heneicosahexaene was the major hydrocarbon together with a small amount of squalene.
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