Stereoselective deamination of (5′ RS)-5′-methyl-2′,3′-isopropylidene adenosine catalyzed by adenosine deaminase: preparation of diastereomerically pure 5′-methyl adenosine and inosine derivatives

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Adenosine deaminase from calf intestine catalyzes the stereoselective deamination of (5′ S)-5′-methyl-2′,3′-isopropylidene adenosine to the corresponding inosine derivative, so that by enzymatic deamination of (5′ RS)-5′-methyl-2′,3′-isopropylidene adenosine, the diastereomerically pure (5′ S)-inosine and the unreacted (5′ R)-adenosine derivatives can be efficiently prepared.