Stereoselective 3 + 2 and stereospecific 2 + 2 cycloaddition reactions of alkenes and quinones

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mM NaCl and 25 mM tris-acetate at pH 7.0.14 After 0.5 h incubation at 24 OC, 4 mM dithiothreitol was added to initiate strand cleavage. The reaction was allowed to continue for 6 h (24 C). The double strand cleavage products were separated on a 0.4% vertical agarose gel which resolves DNA segments up to 25 kbp in size (Figure 3). Therefore, DNA uniquely labeled at the R and