Abstract
Aim:Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative medicine. One major difficulty in establishing a MSC expansion protocol is the large volume of bone marrow (BM) required. We studied whether cells trapped within a collection bag and filter system could be considered as a source of MSC.Results:From the 20 BM collection bag and filter systems, we recovered an average of 1.68 × 108 mononuclear cells, which is the equivalent to 60 ml of filtered BM. Mononuclear cells were expanded ex vivo to 17 × 106 MSC, with purity shown by a CD44+, CD105+, CD90+ and CD73+ immunophenotype, a reduction of 20% proliferating cells in a mixed lymphocyte reaction and also the ability of adipocyte differentiation.Conclusion:Long-term MSC cultures were established from the usually discarded BM collection bag and filter, maintaining an appropriate phenotype and function, being suitable for both investigation and clinical settings.
Highlights
Mesenchymal stromal cells (MSC) are a promising tool for both cellular therapy and regenerative medicine. Their ability to modulate the immune system in a HLA-independent manner makes every MSC donor a universal match
We describe here for the first time an alternative adipogenic media, relying on hospital pharmacy-available drugs capable of inducing adipogenesis
The routine recovery of such cells in reference HSCT centers could, in a way similar to a public cord-blood bank, benefit the scientific community
Summary
Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative medicine. We aim to evaluate whether MNC can be isolated from the BM collection bag/filter and expanded ex vivo into functional MSC, with potential for post-transplant cellular therapy. We aim to expand MSC from the MNC recovered from the collection bag/filter system with an optimized protocol under GMP conditions and fully characterize the final product, performing trilineage differentiation and proliferation assays, in order to validate this as a source of MSC
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