Steady-state concentration distribution of artificial receptor and target analyte in plasticized PVC membrane between solutions differing in target analyte concentration.

Abstract

A barbiturate receptor has proven effective in improving selectivity in solid-phase microextraction of barbiturates when doped into plasticized poly(vinyl chloride) (PVC). It would be beneficial to have selective extractions for any given organic species; however, the receptors do not exist. They will be found by screening of libraries of potential receptors; thus, a screening method is needed. It is important to screen the receptors in the medium in which they will work: plasticized PVC. We hypothesize that we can make receptors move in solution in response to the presence of a solute to which they bind. This work examines whether we can establish a sufficient free energy gradient for a good receptor to move to a predetermined place in space. A difference in the barbiturate solute (substrate or guest) concentration in solutions bathing the two sides of a plasticized PVC membrane containing the barbiturate receptor (or host) creates a spatial concentration gradient of the substrate in the membrane. This causes the receptor's chemical potential to vary across the membrane. Upon binding to the analyte, the receptor undergoes a local activity drop, which decreases its free energy. This process produces a flux of receptor to accumulate at place where there is a high substrate concentration. A concentration gradient of substrate can be maintained across the membrane at steady state. In membranes for which the formation of the complex is favored, the receptor responds to the gradient of substrate. In membranes for which binding is not favored, a gradient of substrate is completely ignored by the receptor. Thus, the receptor does respond to the gradient but only if the concentration gradient of guest corresponds to a chemical potential gradient.