Abstract

Abstract   Sub clinical mastitis in buffalo is considered as multi-factorial disease, which leads to huge economic losses by reducing the quality and quantity of the milk. It does not lead to any change in phenotypic expression, so diagnosed through various tests as California mastitis test, Somatic cell count, Electrical conductivity test etc. Although bacteria are considered to be the main causative agent for mastitis, beside this farm management practices are found to be associated with the disease. In the present study, the pathogens related to the occurrence of the disease were examined via bacteriological culture of milk sample of the animal. Animal wise  prevalence of sub clinical mastitis was found to be (20.4%), out of this 7.8%, 9.8%, 2.8% were found to be effected with latent mastitis, specific mastitis and nonspecific mastitis respectively .On the basis of individual quarter, the highest prevalence was in LH (33.9%), followed by RH (24%) and RF (22.4%) and the least prevalence in LF (14.5%). The microorganisms isolated from sub clinical mastitis constituted Staphylococci (39%), Streptococci (31%), Corynebacterium (25%) and E. coli .(5%).Farm management associated determinants (Type of feed, type of floor, education of the owner, feeding after milking, type of labor) were  studied in association with sub clinical mastitis. Out of these education of the owner , type of labor and feeding after milking were found to be significantly associated with disease occurrence (p<0.05). While looking at the animal determinants in association with sub clinical mastitis in the present investigation buffaloes with higher parity showed more risk of developing sub clinical mastitis. So the present investigation helps to understand the various risk factors for the occurrence of sub clinical mastitis in water buffalo in Doaba region of Punjab.

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