Statistical optimization of lipase production from oil mill effluent by Acinetobacter sp. KSPE71

Abstract

The present study investigated the valorisation of oil-rich residues of coconut oil mill effluent (COME) as a potential growth medium for the microbial production of extracellular lipase. The bacterial species isolated from oil mill effluent, Acinetobacter sp. KSPE71 was tested for its efficiency to grow and produce lipase in undiluted COME and 0.2 % yeast extract and 0.2 % NH4Cl supplemented COME. In this connection, the process parameters such as pH, temperature, agitation speed, and inoculum size were optimized to maximize the production using a central composite design in the Response surface methodology. At the optimized state of pH 7.5, 35?C, 150 rpm with 0.6 % inoculum size, a maximum of 3.95 U mL-1 activity was obtained, four-fold higher than the basal condition. At this stage, 73 % of the lipid content was degraded. The present work results imply that the oil mill effluent can be used as a cheaper production medium for lipase and the new isolate Acinetobacter sp. KSPE71 as a potential lipase producer. The degradation of oil waste along with the production of the valuable product has multiple advantages of cost reduction of lipase and environmental concern.