Abstract
In the present study, the decolorization of Golden Yellow PRA by Citrus reticulate var. kinnow peroxidase (GYPRA) was investigated. Optimization of important process parameters viz. pH, temperature, enzyme dose, concentrations of H2O2, and dye was done by applying one-factor-at-a-time and response surface methodology. Through OFAT analysis, maximum decolorization (91.77%) of the dye was observed with pH 2.0, temperature 40°C, enzyme dose 24 U/mL, H2O2 concentration 0.375 mM, and Golden Yellow PRA concentration of 100 mg/L. The effect of mediators was also investigated and 0.05 mM of vanillin caused 95.98% decolorization, within just 5 min. The presence of metal ions did not exert any drastic influence on the process of decolorization, ensuring it suitable for industrial applications. For statistical optimization, three factors of pH, enzyme dose, and dye concentration were selected. Then, response surface analysis was conducted through central composite design and a second-order polynomial model (0.9894) was generated. ANOVA analysis of this model indicated that the pH, enzyme dose, dye concentration and their interaction were the most significant in decolorization of GYPRA. Optimum condition through RSM was found to be the pH of 3, enzyme dose 26.77 U/mL, and dye concentration of 86.88 mg/L, for enhanced decolorization.
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