Abstract
The article presents an assessment of the dynamics of changes in the content of the marker of collagenolysis – free oxyproline in the homogeniate of soft tissues and bone in experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on these indicators. The aim of this study was to determine the role of cytokinogenesis and the effect of flavonol on it in the pathogenesis, development and course of experimental periodontitis. During the experiment, a fragment of the mandible was taken from the animals, from which the soft tissues and bone were carefully separated. The state of collagen was determined by the content of free oxyproline in the soft and bone tissues. The concentration was determined according to the calibration graph and expressed in μmol/g. The results of studies of the indicators of the state of biopolymers of connective tissue structures of periodontium on the 7th, 14th and 30th day of experimental bacterial-immune periodontitis and after its correction with flavonol (from the 7th to the 14th day of the experiment) are presented. The data on the nature of changes in the content of collagen monomers in the process of formation of the inflammatory focus in the periodontal complex are given. During the acute phase of the inflammatory process in rats there was revealed a slight increase in blood free oxyproline in bone homogenate and homogenate of soft periodontal tissues, on the 14th day the dynamics continued to increase, at a later stage of the experiment, namely on the 30th day, increase in bone resorption continued as compared to the 7th and 14th day. During the correction of disorders resulted from the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14th day. The use of flavonol quercetin, which, by affecting immune processes, limited the inflammatory response in periodontal tissues and stabilized collagenolysis processes in periodontal tissues was manifested by a decrease in free oxyproline in bone and soft tissue homogenates of experimental animals.
Highlights
State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol
Застосування флавонолу кверцетину, який, впливаючи на імунні процеси, обмежував запальну реакцію в тканинах пародонта і призводив до стабілізації процесів колагенолізу в тканинах пародонтального комплексу, що приводило до зниження вільного оксипроліну в гомогенаті кістки та м’яких тканин піддослідних тварин
Experimental animals were divided into 5 groups: I – intact animals (n=10); ІІ – animals with simulated experimental periodontitis, 7th day of the study (n=8); III – animals with simulated experimental periodontitis, 14th day of the study (n=8); IV – animals with simulated experimental periodontitis, 30th day of the study (n=8); V – animals with simulated experimental periodontitis, 14th day of the study, treated with quercetin (n=8)
Summary
State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol. During the correction of disorders resulted from the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14th day. У статті показана оцінка динаміки змін вмісту показника маркера колагенолізу – вільного оксипроліну в гомогенаті м’яких тканин та кістки при експериментальному бактеріально-імунному пародонтиті та з'ясування впливу флавонолу кверцетину на ці показники. При корекції порушень у результаті розвитку цього патологічного процесу відбувалось зниження рівня вільного оксипроліну в кістковому гомогенаті та гомогенаті м’яких тканин пародонта нижньої щелепи, порівняно з такими показниками на 14-у добу, які не отримували кверцетину. Despite the increase in recent years in the arsenal of drugs for the treatment of periodontitis (new antiseptics and other powerful local antimicrobials, herbal medicines, anti-inflammatory and wound healing agents, etc.), the problem of periodontal diseases and their treatment remains topical and timely for theoretical and practical medicine
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
