State of biochemical homeostasis in women with course of pregnancy complicated by miscarriage

Abstract

The objective: estimate oxidative-antioxidant homeostasis in women with pregnancy course complicated by miscarriage. Materials and methods. 90 women in ІІ and at the beginning of ІІІ trimesters of pregnancy were examined. In order to estimate peculiarities of biochemical homeostasis in women with pregnancy course complicated by miscarriage there were studied 44 women with pregnancy course complicated by miscarriage (the main group) and 46 women without clinical manifestations of miscarriage during pregnancy (comparison group). Withdrawal criteria were diseases of cardiovascular, urinary systems and endocrine pathology. Average age of pregnant women in the main group was 27.3±1.5 years and 27.4±1.1 years – in comparison group (p>0.05). Markers of oxidative modification of proteins (OMP) in blood serum were analyzed by spectrophotometric method. State of antioxidative system of protection was determined in accord with level of activity of superoxide dismutase, сatalase, glutathione reductase, glutathione peroxidase and glutathione-S-transferase. Variational and statistical processing of results was made using STATISTICA 13 – license standard application program packages for multidimensional statistical analysis. Results. During study of oxidation-reduction processes in pregnant women who had pregnancy complicated by miscarriage there was revealed that level of OMP exceeded the similar indicators among women without clinical manifestations of miscarriage during current pregnancy (р<0.05). Investigations of superoxide dismutase revealed statistically significant (р<0.05) decrease of activity of enzymes in women with pregnancy course complicated by miscarriage compared to pregnant women without such complication. It should be noted that statistically significant (p>0.05) difference between groups during analyzing of catalase activity was not revealed. Studies of condition of thiol-disulfide system namely activity of erythrocytic glutathione reductase and glutathione peroxidase indicate decrease of their level in women with pregnancy course complicated by miscarriage compared to women without such complication but statistically significant difference (p>0.05) was not found. Conclusion. Analysis of biochemical investigations shows that even in conditions of keeping and progression of pregnancy in women with complication such as miscarriage the antioxidant protection is shifted along with activation of peroxidation processes. Such features are the manifestation of oxidative stress in the present group of pregnant women along with deprivation of enzymic and non-enzymic links of protective antioxidant system. Key words: pregnancy, complications of pregnancy, miscarriage, oxidative modification of proteins, antioxidative system of protection.