Abstract
Many soil microorganisms in nature induce carbonate precipitation, which is used in soil improvement to reduce the pollution of soil environment by traditional chemical improvement. With the goal of expanding the geotechnical applications of biomineralizing bacteria, this study investigated the characteristics of a newly identified strain of soil bacteria, including its mineralization ability, its effect on Aeolian sandy soil, and biomineralization crust effect in the desert field test. The autogenous mineralized strain was isolated and purified from desert Aeolian sandy soil, and the strain was identified as Staphylococcus using 16SrRNA sequence homology. It is a kind of mineralized bacteria seldom used in geotechnical engineering at present, and it was found to have good mineralization ability; the living conditions of Staphylococcus were optimized. Under the determined amount of bacteria liquid, the amount of calcium carbonate precipitation after the bacteria liquid reacted with different volumes of calcium source solution was studied to determine the appropriate ratio of bacteria liquid and calcium source solution and the molar amount of calcium source solution. This study also investigated the effect of mineralization on the strength of sand and determined the particle size range of sand and suitable bacteria concentration that Staphylococcus can effectively stabilize. Field test results verified desert autogenous Staphylococcus had a good mineralization effect on sand. It was suitable for Aeolian sandy soil crust and improvement. In this paper, the autogenous strain was applied to the surface of the desert for the first time to form an in situ microbial mineralization crust. The research results can provide a theoretical basis for the better application of autogenous strain in the desert.
Highlights
Microbial mineralization is a common phenomenon in nature and it has attracted great attention in the field of civil engineering [1]
As Desert soil is a good carrier for microorganisms, the mineralization technique was applied to the field mineralization experiment in the desert, the calcium carbonate crust was induced by the isolated mineralization strain, and the stability of the crust was evaluated, which enables floating dune to be semifixed and fixed, hinders wind erosion, and fundamentally blocks the source of sandstorm. is method overcomes soil pollution by foreign bacteria, and improves the effectiveness of mineralization, and provides a theoretical basis for future popularization into using autogenous bacteria in desert soil solidification and crust technology
CG1 particle size was less than 5 mm (0.075∼5 mm), with most particles between 0.25 and 0.5 mm and a median particle size of 0.7 mm; the natural bulk density of CG1 was 1.578 g/cm3 and the soil particle density was 2.63 g/cm3. e coefficient of uniformity (Cu) of CG1 was 4.96, and its coefficient of curvature (Cc) was 1.529. e CG2 sample was taken from the Ulan Buh Desert in the Inner Mongolia Autonomous Region of China. e shallow layer of the desert is rich in water resources, the underground water level is buried 5∼8 m deep, there are many flowing and semifixed dunes, and the landform is complex
Summary
Staphylococcus Derived from Desert and Its Solidified Ability on Aeolian Sandy Soil. Received 3 August 2020; Revised 13 September 2020; Accepted 28 September 2020; Published 28 October 2020. With the goal of expanding the geotechnical applications of biomineralizing bacteria, this study investigated the characteristics of a newly identified strain of soil bacteria, including its mineralization ability, its effect on Aeolian sandy soil, and biomineralization crust effect in the desert field test. E autogenous mineralized strain was isolated and purified from desert Aeolian sandy soil, and the strain was identified as Staphylococcus using 16SrRNA sequence homology It is a kind of mineralized bacteria seldom used in geotechnical engineering at present, and it was found to have good mineralization ability; the living conditions of Staphylococcus were optimized. Field test results verified desert autogenous Staphylococcus had a good mineralization effect on sand It was suitable for Aeolian sandy soil crust and improvement. The autogenous strain was applied to the surface of the desert for the first time to form an in situ microbial mineralization crust. e research results can provide a theoretical basis for the better application of autogenous strain in the desert
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