Abstract
Abstract The amino acid sequence of the nuclease produced by the Foggi strain of Staphylococcus aureus is being determined as part of a study comparing it with V8 strain nuclease. Amino acid analyses and nuclear magnetic resonance spectroscopy have suggested that Foggi strain enzyme contains 4 histidine residues, in contrast to the 3 known to be present in the V8 strain material. In the course of analyzing the cyanogen bromide fragments of Foggi nuclease, a 13-residue tryptic peptide was isolated which is identical with the corresponding V8 peptide except for replacement of the leucine residue at position 124 by histidine. In addition, the cyanogen bromide fragments are assigned to their positions in the chain with the help of the overlapping series of fragments of nuclease-T, this being an enzymatically active product of limited trypsin digestion of nuclease composed of two large peptide fragments held together by noncovalent interactions. It is concluded that the two nuclease molecules are very similar and that the replacement of leucine 124 (V8) by histidine (Foggi) is compatible with an active conformation.
Published Version
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