Abstract
During embryogenesis, the expression of mammalian stanniocalcin (STC1) in the appendicular skeleton suggests its involvement in the regulation of longitudinal bone growth. Such a role is further supported by the presence of dwarfism in mice overexpressing STC1. Yet, the STC 1 inhibitory effect on growth may be related to both postnatal metabolic abnormalities and prenatal defective bone formation. In our study, we used an organ culture system to evaluate the effects of STC on growth plate chondrogenesis, which is the primary determinant of longitudinal bone growth. Fetal rat metatarsal bones were cultured in the presence of recombinant human STC (rhSTC). After 3 days, rhSTC suppressed metatarsal growth, growth plate chondrocyte proliferation and hypertrophy/differentiation, and extracellular matrix synthesis. In addition, rhSTC increased the number of apoptotic chondrocytes in the growth plate. In cultured chondrocytes, rhSTC increased phosphate uptake, reduced chondrocyte proliferation and matrix synthesis, and induced apoptosis. All these effects were reversed by culturing chondrocytes with rhSTC and phosphonoformic acid, an inhibitor of phosphate transport. The rhSTC-mediated inhibition of metatarsal growth and growth plate chondrocyte proliferation and hypertrophy/differentiation was abolished by culturing metatarsals with rhSTC and phosphonoformic acid. Taken together, our findings indicate that STC1 inhibits longitudinal bone growth directly at the growth plate. Such growth inhibition, likely mediated by an increased chondrocyte phosphate uptake, results from suppressed chondrocyte proliferation, hypertrophy/differentiation, and matrix synthesis and by increased apoptosis. Last, the expression of both STC1 and its binding site in the growth plate would support an autocrine/paracrine role for this growth factor in the regulation of growth plate chondrogenesis.
Highlights
Stanniocalcin (STC)2 is a glycoprotein first identified as a secretory product of the corpuscles of Stannius, an endocrine gland unique to bony fish [1, 2]
Because the rate of longitudinal bone growth depends primarily on the rate of growth plate chondrogenesis, we evaluated the effects of recombinant human STC (rhSTC) on chondrocyte hypertrophy/differentiation and chondrocyte proliferation
The addition of rhSTC to the culture medium of rat metatarsal bones caused a concentration-dependent suppression of their linear growth, suggesting a direct inhibitory effect of STC1 on mammalian longitudinal bone growth
Summary
Stanniocalcin (STC)2 is a glycoprotein first identified as a secretory product of the corpuscles of Stannius, an endocrine gland unique to bony fish [1, 2]. We cultured rat metatarsal bone rudiments in the presence of recombinant human STC (rhSTC) and assessed its effects on metatarsal linear growth, growth plate chondrocyte proliferation and hypertrophy/differentiation, cartilage matrix synthesis, and apoptosis.
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