Standarisasi dan Analisis Kandungan Flavonoid Total Daun Pulai (Alstonia shcolaris L.)

Abstract

The island plant (Alstonia scholaris) is one of the plants that is often used for traditional medicinePulai plant (Alstonia scholaris L.) is often used as a traditional medicine to treat fever, itch, malaria, and diarrhea. The secondary metabolite compounds obtained from this plant are flavonoid, tannin, and steroid. This study aims to determine the specific and non-specific parameters and the total flavonoid levels of Pulai's 70% ethanol extract. The standardization of medicine plant extract is needed as an effort to traditional medicine quality control. The standardization test result for specific parameter showed that the organoleptic extract was a dark green and thick extract, bitter, and had a distinctive smell, containing water soluble content (20.14% ±0.1649) and ethanol soluble content (9.495%±0.0928). The standardization for non-specific parameter showed water content (9.17%±0.02055), total ash content (1.76%±0.00547), acid insoluble ash content (2.82%±0.01257), and drying shrinkage (5.08%± 0.04636). Meanwhile, the analysis of flavonoid level of Pulai's 70% ethanol extract fraction was done at the wavelength of 438.36 nm and the absorbance value of 0.0011. The total flavonoid content was analyzed using quercetin standard linear equation of y=0.0643x + (-0.0232) with the correlational coefficient (R)- 0.9974, and the total flavonoid content of 3.77%.