Abstract

Flavonoids are a group of phytochemical compounds commonly found in plants. Jatropha curcas L. And Jatropha gossypiifolia L. Are plants belonging to the same family, Euphorbiaceae. Both of these plants have significant potential, especially in health. This research aims to determine the comparison of total flavonoid levels in methanol extracts of Physic Nut (Jatropha curcas L.) and Cotton-Leaf Physic Nut (Jatropha gossypiifolia L.) leavs using U-Vis spectrophotometry method. This research utilizes both qualitative and quantitative analysis methods. The qualitative analysis involves phytocemical screening and Thin-Layer Chromatography analysis using an eluent of n-Hexane ethyl acetate with a ratio of (8:2). The quantitative analysis in this research utilized the UV-Vis spectrofotometry method at a wavelength of 435 nm with quercetin as the standard comparator. The results indicate that the average total flavonoid content in 100 mg of methanol extract from Physic Nut (Jatropha curcas L.) leaves is 5,75% w/v. Meanwhile, in the methanol extract from Cotton-Leaf Physic Nut (Jatropha gossypiifolia L.) leaves, it is 7,20% w/v. Based on Independent Sample T-test statistical analysis, the total flavonoid content in the methanol extract from Cotton-Leaf Physic Nut leaves is significantly higher than that in the methanol extract from Physic Nut Leaves, indieating a significant difference in the average values of two samples (p-value0,05).

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