STANDARDIZATION OF PULIYAARAI NEI: A SIDDHA POLY HERBAL FORMULATION

Abstract

Siddha is one of the Indian systems of medicine which is widely practiced in Tamil Nadu, India. Siddha insists to lead a healthy life both physically and mentally. Siddhars have classified 4448 disease, based on the three humoral pathologies. Among them Ratha Moolam (Bleeding piles) is classified under Eruvaimulainoi. Puliyaarai Nei has indication for Ratha Moolam (Bleeding piles) as per siddha text Aathmaratchamirthamennumvaithiyasaarasangiragam (siddha vaithiyathinaathinool). The drug is prepared as per the method mentioned in the Siddha literature. Drug standardization of herbal formulations is essential for their acceptance in this scientific medical world. In this article Physicochemical, Phytochemical, biochemical analysis and other standardization methods of one such polyherbal medicated ghee Puliyaarai nei are discussed. The organoleptic characteristics, pH, Iodine number, Saponification value, Peroxidase value, Acid value were studied. The Qualitative study was done in biochemistry and the results are as follows, puliyaarai nei showed the Presence of carbonate, aluminium, iron, zinc, calcium, magnesium, ammonium, mercury, starch, arsenic and alkaloid. Preliminary phytochemical screening was done for the presence of flavonoids, steroids and betacyanin. HPTLC finger printing, Heavy metal analysis, Pesticide residues, Microbial contamination were studied as per the Pharmacopoeial laboratory standards of Indian medicine. In results, it was found that the specified drug was containing various phytochemicals and is free from microbial contamination, and pesticide residues. The heavy metals such as Arsenic, Mercury, Cadmium and Lead are not detected.