Standardization of protocol for in vitro propagation of banana (Musa sapientum)

Md Fakhrul Hasan,Jahanara Parvin,Farhana Hoque,Mahbub Robbani

doi:10.3329/jbau.v16i1.36477

Md Fakhrul Hasan, Jahanara Parvin + Show 2 more

Open Access

https://doi.org/10.3329/jbau.v16i1.36477

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Abstract

An experiment was conducted at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University during the period from August 2016 to April 2017 to standardize the protocol for in vitro propagation of banana. The experiment was laid out in completely randomized design with four replications. Three to four months aged field grown rhizome attached shoots were used as explants and cultured on MS medium with different concentrations and combinations of BAP (6-benzylamino purine), BAP + KIN (Kinetin) + NAA (Naphthalene Acetic Acid) and IBA (Indole-3- Butyric Acid) + IAA (Indole-3- Acetic Acid) to observe their efficacy on single shoot initiation, shoot multiplication and root formation respectively. Minimum number of days required for shoot initiation (9.07) with highest shoot initiation percentage (91.14) and the longest shoot (2.23 cm) was found in MS medium supplemented with 5.0 mg/L BAP. On the other hand, highest shoot multiplication percentage (80.99) with maximum number of shoots per explant (4.47), the highest length of shoots (4.17 cm) and maximum number of leaves (4.04)was observed in MS medium supplemented with 4.0 mg/L BAP + 2.0 mg/L KIN + 2.0 mg/L NAA. In case of root regeneration, the best results on days required for root initiation (9.00), the highest root initiation percentage (85.05), maximum number of roots per plantlet (5.83) and the highest length of roots (4.17 cm) was obtained in MS medium supplemented with1.5 mg/L IBA + 0.5 mg/L IAA. After 5-7 days of hardening in room temperature, established plantlets were ready for plantingJ. Bangladesh Agril. Univ. 16(1): 27-30, April 2018

Highlights

Banana is a perennial herbaceous monocot which is originated from Malaysia through a complex hybridization process (Novak, 1992)
Standardization of in vitro propagation of banana debris and outer tissues were removed until the explant measured about 2.0–3.0 cm in length and 2.0 cm width with the help of sharp knife
The research activities of in vitro propagation of amritasagar banana (Musa sapientum) using different growth regulators were developed under three experiments that were done separately

Summary

Introduction

Banana is a perennial herbaceous monocot which is originated from Malaysia through a complex hybridization process (Novak, 1992). High yielding varieties are unavailable and the traditional clonal propagation method appears to be unable to supply the increasing demand for disease free and healthy planting materials of banana and it is season dependent (Hanumantharaya et al, 2009). To overcome this problem, tissue culture or in vitro propagation offers an alternative over traditional propagation methods facilitating, large scale production of disease-free planting materials all year round keeping, physiological uniformity of the plants from using only a few explants (Abeyaratne and Lathiff, 2002; Waman et al, 2014). The present study was undertaken to evaluate the propagation rate of M. sapientum under the influence of PGRs as well as large scale production of banana

Materials and Methods

Results and Discussion

Conclusion