Abstract
Amukkara curanam, a Siddha formulation, currently used in all types of gastric disorders, rheumatic pain, insomnia and sexual insufficiency, was investigated for the estimation of the marker compounds, withaferine A and piperine contents in a prepared standard formulation and a commercial formulation by using HPTLC method of analysis. The two formulations were subjected to methanol, ethyl acetate and chloroform extractions by using Soxhhlet apparatus The chromatogram was developed using chloroform: methanol (8.5:1.5 v/v) and toluene: ethyl acetate (7:3 v/v) as mobile phases for the estimation of withferine A and piperine respectively. The detection and quantification were performed at a wavelength of 220 nm for withaferine A and 254 nm for piperine. The linear regression analysis of calibration plots of withferine A and piperine exhibited linear relationship in the range of 5-15 microg and 50-150 ng respectively, while the % recovery was found to be 94.52% w/w of withaferine A and 98.73% w/w of piperine, thus proving the accuracy and precision of the analysis. Methanol and ethyl acetate were found to be the suitable solvents for the extraction of withaferin A and piperine respectively. The withaferine A content in standard formulation was found to be much higher in all the three extracts than that of the commercial sample. However, the piperine content in all the three extracts of standard formulation was slightly lower than the respective extracts of commercial formulation. The proposed HPTLC method was found to be rapid, simple and accurate for quantitative estimation of withferine A and piperine in different formulation extracts.
Highlights
Herbal medicine has witnessed a renaissance among the customers throughout the world
Good correlation was obtained between standard and samples overlay spectra of withaferine A (Figure 11) and piperine.(Figure 18) Calibration curves were prepared by plotting concentrations versus average area of the peak.(Fig 3 and Fig 4)
The piperine content in methanol, ethyl acetate and chloroform extracts were found as 0.347%w/w, 0.363%w/w and 0.342%w/w respectively in Standard Formulation (SF) sample and 0.377%w/w, 0.383%w/w and 0.375%w/w respectively in Commercial Formulation (CF) sample of Amukkara curanam
Summary
Herbal medicine has witnessed a renaissance among the customers throughout the world. In spite of the large number of herbal formulations available in the market, standards for their quality are yet to be laid for many of them (Eapen Saumy M.S, et al 2002; Patel K.N, 1996). It is imperative to develop fast, sensitive and accurate quality control tests for Siddha formulations which will be in alignment with modern technology. The quality of herbal medicine, that is, the profile of the constituents in the final product has implication in efficacy and safety. A scientific investigation was undertaken to evaluate Amukkara curanam
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: African Journal of Traditional, Complementary and Alternative Medicines
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
