Standardised Sampling Approach for Investigating Pathogens or Environmental Chemicals in Wild Game at Community Hunts.

Abstract

Simple SummaryWild game can host pathogens capable of infecting humans, livestock, and companion animals or accumulate environmental chemicals that may be transferred via food of animal origin. For food safety research, as well as for various other scientific purposes, the provision of a sufficient number of samples without unnecessary disturbance or killing of animals is a major limitation. With the presented approach, it was possible to obtain samples from game animals killed as part of standard ungulate management practice. Examples of organs, tissues, and other matrices that have been used in wild ungulate studies in Europe and that may be obtained through this approach are summarised as well. The basis of this approach was a framework agreement with the BImA, whereby federal forest officials carried out sampling with the help of hunters at drive hunts from 2017/18 to 2020/21 in Brandenburg, Germany. Numerous samples from four ungulate species were obtained. The number of sampled animals per hunt differed between hunting districts and hunting seasons. Districts with higher hunting bags also promise higher sampling success. This approach can serve as the basis for long-term monitoring of animal and public health threats associated with wildlife and is adaptable to other regions.Wildlife may host pathogens and chemicals of veterinary and public health relevance, as well as pathogens with significant economic relevance for domestic livestock. In conducting research on the occurrence and distribution of these agents in wildlife, a major challenge is the acquisition of a sufficient number of samples coupled with efficient use of manpower and time. The aim of this article is to present the methodology and output of a sampling approach for game animals, which was implemented from 2017/18 to 2020/21 at drive hunts in Brandenburg, Germany. The central element was a framework agreement with the BImA, whereby federal forest officials and other hunters collected most of the samples during field dressing. Further samples of game carcasses were obtained by scientists during subsequent gathering at a collection point. Altogether, 3185 samples from 938 wild ungulates of four species were obtained for various studies analysing—in this case—food-borne agents in game animals. Sampling was representative and reflected the proportional distribution of ungulate species hunted in Brandenburg. Hunting district and hunting season strongly influenced hunting bag and hence sampling success. This sampling approach was demonstrated to be a suitable basis for monitoring programs, that can be adapted to other regions.