Abstract
The World Health Organization has encouraged the development of medicinal plant monographs in various countries. The present study therefore aimed at developing pharmacognostic standards for the quality evaluation of Holarrhena floribunda (G. Don) Durand & Schinz, used as anti-infective in folklore medicine. The macromorphological and micromorphological features, physicochemical, phytochemical and thin layer chromatograms of the leaves and stem bark were evaluated using standard methods. An HPLC method was also developed and validated to profile some steroidal alkaloids of the stem bark. The plant has simple, glaborous leaves, broadly lanceolate to ovate in shape and opposite in arrangement. The leaves were hypostomatic with paracytic and anomocytic stomata on the abaxial surface. The flat stem bark is light brown on the inner surface. Three alkaloids were profiled as chemical markers for the quality control of the stem bark of H. floribunda, to aid its correct identification for research and industry.
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