Abstract
A procedure is described for stably transfecting mouse adrenocortical Y1 cells with AT1a and AT1b angiotensin II (AII) receptor subtypes using the lipofectin reagent, DOTAP. The transfected cells are screened by radioreceptor assay using125I-[Sar1,Ile8]AII to select those cells that express high levels of the AII receptors. The establishment of individual transfected Y1 cell lines expressing AT1a and AT1b receptors provides a valuable model system in which to compare the function and signal transduction mechanisms of these two highly homologous AII receptor subtypes.
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