Abstract
Enzyme catalysis has clear advantages in the process of oxidizing glucose to produce gluconic acid. In the enzyme cascade, the improvement of the cascade efficiency is desired but challenging. Graphene oxide (GO) and ZIF-8 composites as enzyme support offer the promising opportunity that not only the cascade efficiency can be improved by control the distance between two enzymes, but also the stability can be improved. Here, a new strategy of GO assisted co-growth of ZIF-8 and enzyme was carried in a one-pot synthesis. Glucose oxidase&catalase immobilized in the ZIF-8/GO composites can obtain 98% residual activity after 15 days of storage with almost no enzyme shedding. The residual activity is still higher than 75% after 5 repeated uses. The presented method of controllable growth of metal organic frameworks on 2D nanosheet can also be extended for renewable energy devices, gas storage and separation of small molecules.
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