Stabilization of a Lipolytic Enzyme for Commercial Application

Simone Antonio De Rose,Jennifer Littlechild,Sukriti Singh,Halina Novak,Dietmar Andreas Lang,Andrew Dowd

doi:10.3390/catal7030091

Simone Antonio De Rose, Jennifer Littlechild + Show 4 more

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https://doi.org/10.3390/catal7030091

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Abstract

Thermomyces lanouginosa lipase has been used to develop improved methods for carrier-free immobilization, the Cross-Linked Enzyme Aggregates (CLEAs), for its application in detergent products. An activator step has been introduced to the CLEAs preparation process with the addition of Tween 80 as activator molecule, in order to obtain a higher number of the individual lipase molecules in the ”open lid” conformation prior to the cross-linking step. A terminator step has been introduced to quench the cross-linking reaction at an optimal time by treatment with an amine buffer in order to obtain smaller and more homogenous cross-linked particles. This improved immobilization method has been compared to a commercially available enzyme and has been shown to be made up of smaller and more homogenous particles with an average diameter of 1.85 ± 0.28 µm which are 129.7% more active than the free enzyme. The CLEAs produced show improved features for commercial applications such as an improved wash performance comparable with the free enzyme, improved stability to proteolysis and a higher activity after long-term storage.

Highlights

Over the last decade, there has been a trend in the industry to move towards more eco-friendly products as a way to boost a company’s “green” credentials, and this is driving economic growth [1].In the laundry industry, many research and development projects have been focused on the creation of novel formulations using less polluting chemicals and more bio-based components including enzymes and natural surfactants
Oxygen Demand (BOD) in the waste stream is negligible [2], which results in clear ecological benefits
Thesemay included numerous non-ionic surfactants, A selection of compounds were identified have potential beneficial effects on the ether compounds, waxes and oils, natural fats, natural detergents, inhibitors and amino activity retained by the Lipex Cross-Linked Enzyme Aggregates (CLEAs) (Table 1)

Summary

Introduction

There has been a trend in the industry to move towards more eco-friendly products as a way to boost a company’s “green” credentials, and this is driving economic growth [1]. Many research and development projects have been focused on the creation of novel formulations using less polluting chemicals and more bio-based components including enzymes and natural surfactants. In western Europe, consumers have shown a preference for liquid laundry detergent rather than powder detergent [3]. Compared to a powder formulation, liquid detergent is more concentrated, less environmentally aggressive and needs less packaging material, which results in a lower carbon footprint for the product. In a typical biological laundry detergent formulation, there are at least three types of enzymes which address different types of stains, which include proteases, amylases, and lipases, but additional

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