Stability Test of Nanostructured Lipid Carriers-Loaded Mefenamic Acid prepared by Microemulsion Technique

Abstract

The objectives of this study were to prepare the nanostructured lipid carriers (NLCs)-loaded mefenamic acid and to study the particle size and zeta potential of the NLCs-loaded mefenamic acid after storage in refrigerator (approximately 2 – 4°C) for 7 days. The preparation method of NLCs-loaded mefenamic acid used the microemulsion technique. All compositions were vigorously mixed with vertex method to obtained the clear microemulsion solution. Tween®80 (surfactant) and 1 – butanol (co – surfactant) were mixed at the ratio of 3:2 to use as the surfactant mixture. Then, it was dispersed in cold water (approximately 2 – 4°C) at different ratio, and the droplet size was reduced by a homogenizer at 6, 000 rpm for 5 minutes and sonicator for 30 minutes to form the NLCs-loaded mefenamic acid. The prepared NLCs-loaded mefenamic acid were kept in refrigerator (approximately 2 – 4°C) for 7 days. After that, the solutions were tested the particle size and zeta potential. The particle size and zeta potential of NLCs-loaded mefenamic acid after storage were in the range of 160-310 nm and -4.00 to -19.00 mV, respectively. It was found that the F4 formula was the best to prepare the NLCs-loaded mefenamic acid. F4 formula composed of the 1%w/w mefenamic acid, 10%w/w water, 80%w/w surfactant, and 10%w/w capric triglyceride, and the dilution ratio was 1:25.