Abstract
Six chromatographic grade soybean isoflavones solutions daidzin, glycitin, genistin, daidzein, glycitein, and genistein were prepared with a preparative high performance liquid chromatography(Shimadzu LC-20AP). The solution stabilities were monitored in changes of concentrations and purities during a one-month period under temperatures of –20℃, 4℃, 25℃, and 60℃, respectively, and during a six-month period under a temperature of 4℃. Variance analysis of the one-month period experimental results indicated that the concentrations of five soybean isoflavones solutions showed no obvious change under 4℃ and 25℃except for genistin solution. No obvious changes in purities for all soybean isoflavones solutions under –20℃, 4°C, and 25℃were observed. Temperatures of 4℃ and 25℃ were suggested for short-term storage of soybean isoflavones solutions. The t-test of the six-month period experimental results indicated that the concentrations of six chromatographic grade soybean isoflavones solutions increased significantly, but the purities of those did not change after 0, 1, 2, 3, 4, 6, 8, 12, 16, and 24 weeks preservation at 4℃. Leakage of methanol from containers of isoflavones solutions was considered to be the course of concentration increment. This research provided technical helps for the production of the soybean isoflavones standard solutions.
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