Abstract
Aqueous ragweed pollen extract (20,000 or 78,000 PNU per milliliter) lose 50 per cent of their antigen E content within five months of storage at 4 °C. Glycerinated extracts and lyophilized ragweed pollen extracts retain their antigen E content for over 15 months with little or no loss. The preservative phenol in aqueous extracts is destructive to antigen E, probably by protein denaturation. The stability of ragweed extracts as measured by antigen E content is markedly enhanced with little loss after 15 month storage at 4 °C. when the extract is prepared as an alum-precipitated suspension.
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