Abstract
Four fractions of the dialyzable components of the aqueous extract of ragweed pollen were prepared and their physicochemical and immunologic properties were examined. All these fractions were skin active with respect to sera from both treated and nontreated individuals. All the antigenic and allergenic determinant groups of the dialysate fractions were found to be present in the nondialyzable components of the whole aqueous extract. Contrary to previous conclusions, it was demonstrated by cross-neutralization studies that new reagin(s) directed exclusively against components of the dialysate is not produced by hyposensitization treatment with the whole aqueous extract. Rather, any increase in reagin concentration caused by hyposensitization is considered to be the result of a rise in the concentration of reagin (s) already being produced by the allergic individual.
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