Abstract
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yellow emission profiles. These variants allow in vivo imaging where original iGluSnFR was too dim, can resolve glutamate transients in dendritic spines and axonal boutons, and permit kilohertz imaging.
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