Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.

Jonathan S Marvin ,Victor J Depiero,Ariana N Tkachuk,David Fitzpatrick,Susanne Schoch,Abbas Kazemipour,Dirk Dietrich,Johannes Alexander Müller,Francisco José Urra Quiroz,Adam W Hantman,Edwin R Chapman,Loren L Looger,Edward Cai,Kaspar Podgorski,Huan Bao,Bart G Borghuis,David A Digregorio,Benjamin Scholl,Justin P Little,Nelson Rebola,Samuel S.‐H Wang ,Dana E Wilson

doi:10.1038/s41592-018-0171-3

Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.

Jonathan S Marvin , Victor J Depiero + Show 20 more

Open Access

https://doi.org/10.1038/s41592-018-0171-3

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Journal: Nature Methods	Publication Date: Oct 30, 2018
Citations: 342	License type: unspecified-oa

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, University of Louisville, Max Planck Florida Institute for Neuroscience, University of Bonn, Stanford University, Institut Pasteur, Dynamic Neuronal Imaging, University of Wisconsin–Madison, Johns Hopkins University, New York University Langone Medical Center, Princeton University

#Axonal Boutons #Dendritic Spines + Show 8 more

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Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter, detect sub-micromolar to millimolar glutamate, and have blue, cyan, green, or yellow emission profiles. These variants allow in vivo imaging where original iGluSnFR was too dim, can resolve glutamate transients in dendritic spines and axonal boutons, and permit kilohertz imaging.

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