Abstract
Eggplant (Solanum melongena L.) is an important crop in terms of economic and genetic significance in tropical and subtropical regions. The Indian Institute of Horticulture Research (IIHR) eggplant breeding program produced five genotypes with increased yield and excellent producer acceptance. Molecular profiles of these genotypes were developed using 39 EST-SSR primers for maximum discrimination and repeatability at 35 loci. In total there were 181 alleles with the number of alleles per locus ranging from 9.0 (EEMS 24) to 3 (EEMS 46 and EEMS 20), with a mean of 5.02 alleles per locus ranging from 85 to 300 bp. Polymorphic information content of the markers ranged from 0.343 to 0.794. Expected heterozygosity ranged from 0.560 to 0.880 and the probability of identity ranged from 0.010 to 0.376. Pair-wise comparison of microsatellite data led to development of an unweighted pair group method with arithmetic mean dendrogram and DNA barcodes for easy and accurate identification of eggplant genotypes with combinations of their morphological traits. The results indicate that ‘IIHR-3’ and ‘IIHR-7’ originated from a single source and ‘Arka Anand’ is a cross between ‘IIHR-3’ and ‘SM-6-6’, indicating a good fit with genetic similarity values. The DNA barcode fingerprint can be deposited and used for clear identification of eggplant genotypes and to address intellectual property rights–related issues.
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