Abstract
Surface plasmon resonance (SPR) is an established method for sensing analytes by monitoring changes in the plasmon dispersion relation (PDR) at the interface of a thin metal film and a fluid. When SPR is used in sensors for specific analytes, the changes in the plasmon dispersion relation of interest are generated by the binding of analytes to receptors immobilized to the metal film. However, changes in the PDR can also be generated by changes in the index of refraction of the bulk solution containing the analytes via changes in composition or temperature. Thus, there exist inherent systematic errors in SPR based chemical sensing when temperature and/or concentration conditions are not carefully controlled. We have demonstrated the efficacy of a single, simple, and inexpensive method for simultaneously discriminating both effects from those of binding and/or debinding of analytes with a two-element SPR sensor array. Although two-element SPR arrays have been used before, their ability to simultaneously discriminate out both thermal and bulk-composition (TAB) effects (in both SPR spectroscopic and spectrophotometric schemes) has not been previously addressed. We show an example of a relatively inexpensive SPR biosensor instrument using a compensator element and its stability over a period of days. This demonstration has implications for the development of reliable SPR based chemical sensors for environmental and remote sensing applications.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.