Abstract
Great scientific strides have been achieved upon discovering the great plasticity of adult mesenchymal stem cells. Such stem cells can be harvested from various sources, including subcutaneous adipose tissue which represents an almost ideal cellular reservoir. Cell therapies using adipose‐derived stem/stromal cells (ASCs) are being conducted in an increasing number of clinical trials such as osteoarthristis and congestive heart failure. The other field reaping the benefits of using ASCs is tissue reconstruction. The multipotency of these cells combined with different engineering strategies result in the in vitro production of a wide variety of human tissues. My research team is using ASCs as building blocks for the production of human tissue substitutes, including adipose tissue itself and skin. They feature a rich extracellular matrix produced by the mesenchymal cells themselves upon ascorbic acid stimulation, recreating a very physiological 3D environment. The reconstructed adipose tissues are functional and secrete important cytokines and growth factors such as leptin, Ang‐1, HGF, and VEGF. This secretome is modulated in a dose‐dependent manner following exposure to the inflammatory cytokine TNFα. Also, production of reconstructed skin using ASCs allowed us to investigate the fate of epithelial stem cells in these engineered tissues, before and after grafting. Supported by CIHR and NSERC.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
