Abstract
Spore culture of Nephrolepis falcate (Cav). C. Chr. was undertaken to propagate Nephrolepis falcata in vitro using the spores. Specifically, it aimed to find out the duration of the germination of Nephrolepis falcate spores and observe spore germination and prothallial development. Nephrolepis falcate is a fern plant under family Lomariopsidaceae which is found on shaded areas (Smith et al., 2006). Its rhizome is creeping, frond is simple pinnate, sori round and spores bilateral, monolete. This species was used as ornamental. Thus, there is a need to conduct an in vitro (spore) culture of this plant. In vitro culture of this species revealed a rapid spore germination of 3 days after inoculation. Germ filament formation of one to twelve cells was observed from 3rd to 16th day after inoculation, and prothallial plate formation was noticed 19th day after inoculation. Correspondingly, Nephrolepis falcate (Cav.) C. Chr. spore germination is of Vittaria type and its prothallial development follows Adiantum-type.
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