Abstract
The induction of the proliferative response of B cells by lipopolysaccharide (LPS) and the proliferative activity of T lymphocytes during stimulation by phytohemagglutinin (PHA) in patients with pollinosis, with hypersensitivity to timothy-grass pollen, were studied. Peripheral blood mononuclear cells (MNC) from 17 patients with pollinosis aged from 16 to 40 years, were used. Lymphocytes (MNC) were isolated form heparinized blood by centrifugation and LPS from E. coli serotype 026:B6 in final concentrations was used as B-cell activator. Tritium-thymidine was added 18 h before the end of the culture time. Incorporated radioactivity was counted in a Mark III liquid scintillation beta-counter. The hyperproduction of IgE observed in atopic patients is more likely to be connected with changes in the later stages of differentiation of IgE-synthesizing B-lymphocytes (at the memory cell level) into IgE-producing plasma cells.
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