Spontaneous and action potential-evoked Ca2+ release from endoplasmic reticulum in neocortical synaptic boutons

Abstract

Although the endoplasmic reticulum (ER) is present throughout axons, and IP3 and ryanodine receptors are widely expressed in nerve terminals, whether Ca2+ release from presynaptic stores contributes to action potential (AP)-evoked Ca2+ transients remains controversial. We investigated the release of Ca2+ from ER stores in boutons en passant of neocortical layer 5 pyramidal neurons. A hallmark of these stores is that they spontaneously release Ca2+ at a low frequency. Using a high-affinity Ca2+ indicator, we documented and characterised such spontaneous Ca2+ transients (sCaTs), which occurred at a rate of ~0.2 per min and raised the intracellular Ca2+ concentration ([Ca2+]i) by ~2 µM in the absence of exogenous buffers. Caffeine increased the average frequency of sCaTs by 90%, without affecting their amplitude and decay kinetics. Therefore, presynaptic ryanodine receptors were likely involved. To determine if presynaptic ER stores contribute to intracellular Ca2+ accumulation during repetitive stimulation, we measured [Ca2+]i during 2 s long trains of APs evoked at 10–50 Hz. We found that for frequencies <20 Hz, [Ca2+]i reached a steady state within ~500 ms after stimulation onset. However, for higher frequencies, [Ca2+]i continued to increase with AP number, suggesting that the rate of Ca2+ entry exceeded the rate of clearance. Comparison between measured and predicted values indicates supralinear summation of Ca2+. Block of the sarco/endoplasmic reticulum Ca2+-ATPase reduced the supralinearity of summation, without reducing the amplitude of a single AP-evoked Ca2+ transient. Together, our results implicate presynaptic ER stores as a source of Ca2+ during repetitive stimulation.