Abstract
Radicals formed during oxidative metabolism of ethylhydrazine have been spin-trapped with PBN 1 1 Abbreviations: PBN, α- phenyl- tert - butylnitrone ; DMPO, 5,5-dimethylpyrroline-N-oxide; DETAPAC, diethylenetriaminepentaacetic acid; ESR, electron spin resonance. . The trapped species has been identified as an ethyl radical by comparison of the ESR parameters of the PBN-adduct with (1) those of the analogous adduct formed during CuCl 2-catalyzed oxidation of ethylhydrazine and (2) those reported for the adduct in the literature. The requirement for oxygen and NADPH, and the observation of a typical binding spectrum by difference spectroscopy, suggest the involvement of the cytochrome P-450 enzyme system in radical formation. Similar studies with acetylhydrazine demonstrate the generality of metabolic radical formation from hydrazines, although the radical trapped in this latter instance has not been identified.
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