Abstract
In typical optical tweezers experiments the quality of the trap deteriorates due to optical aberrations caused by the refractive index mismatch at the glass–water interface when trapping deep inside a sample or out of the imaging plane. We investigate this effect experimentally by monitoring the two-photon fluorescence of trapped dye-stained polystyrene spheres. We also partly corrected the aberrations by optimising the fluorescence signal using an adaptive deformable membrane mirror.
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