Abstract
Fractionation with reverse-phase HPLC and electrophoretic analyses revealed that the nuclear basic proteins in sperm of an anuran amphibian, Discoglossus pictus, consisted of weakly basic proteins without histones. Amino acid analyses indicated that none of these proteins were histones or protamines because of the low amounts of Lys and Arg. The predominant protein among those fractionated possessed an unusually high content of His and extremely low amounts of Arg and Lys, indicating that this is a unique nuclear basic protein not reported previously. The induction of a highly decondensed state of sperm nuclei upon their incubation in egg extract was accompanied by the removal of most, if not all, of this His-rich protein from the nuclei.
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