Abstract
Personal identification of two individuals in mixed semen samples in forensic DNA testing in general usually involves analysis using autosomal and Y chromosome short tandem repeats (STRs). Results may exclude unrelated donors but cannot identify individuals. In this study, sperm cell capture based on ABH antigen differences was used to obtain the cells with the single ABO blood type. Immunohistochemical staining using labeled anti-A, anti-B, and anti-H antibodies and the laser microdissection system can be used to enrich sperm with different ABO types in mixed seminal stains from two individuals. Then, PCR amplification and capillary electrophoresis were performed to genotype the STR loci. To some extent, after sperm cell capture based on ABH antigen differences, autosomal STR typing using enriched single blood group cells can be utilized to partially identify different individuals in a mixed seminal stain sample from two individuals.
Highlights
A mixed stain means that the specimen being analyzed was derived from two or more individuals
Differential extraction procedures are used to remove the female composition from the sample, followed by autosomal small tandem repeat (STR) typing
Due to paternal inheritance of the Y chromosome, men belonging to one agnate have the same Y chromosome STR type [7, 8]
Summary
A mixed stain means that the specimen being analyzed was derived from two or more individuals. The sperm cell surface contains ABH antigens, which correspond to individual ABO blood types. Our method may provide an effective selection for the single male STR profile in mixed seminal stains.
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