Abstract
We have used electronic absorption and fluorescence spectroscopy to study binding between a platinum(II) dimethylsulfoxide complex (cis-[Pt(DMSO)2Cl2]) and human serum albumin (HSA), and the effect of complexation on the structure of the protein. We have calculated the binding parameters for binding between cis-[Pt(DMSO)2Cl2] and HSA. We have determined the binding constant KB = (1.2 ± 0.1)·103 M−1 and the Hill coefficient h = 1.03 ± 0.1. We have determined that binding between cis-[Pt(DMSO)2Cl2] and the protein leads to a change in the internal packing of the macromolecule.
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