Spectroscopic, characterization and bioactivity studies of new Ni (II), Cu (II) and Ag (I) complexes with didentate (N,N) donar azo dye ligand

Abstract

To prepare the 2-(4-Bromo phenyl) azo imidazole, 4-Bromo aniline was diazotized in conjunction with imidazole as a coupling component. The complexes of Ni(II), Cu(II), and Ag(I) with (4-BPAI) have been prepared and well characterized using elemental microanalyses (C.H.N), (1H & 13CNMR , infrared, electronic and mass) spectra. The molar conductivity shows that the complexes are electrolytes, but the value of conductivity indicated that the silver (I) complex was non-electrolytes. The X-ray powder diffraction and FE-SEM techniques were used to identify the nano-size particles of both Ag(I) and Cu(II) complexes. The mole ratio [M:L], was also studied which was 1:1 for Ag (I) and 1:2 for the rest of the complexes, and the ligand behaved as a bidentate chelating. The antimicrobial assessments of the ligand and its complexes were conducted against some types of bacteria and fungi. Further, the in silico molecular docking showed effective binding properties of the compounds against the CDK4 target receptor. The cytotoxic activity of the ligand and silver (I) complex were tested against the human Laryngeal carcinoma (AMCHN-8) tumor cell lines.