Abstract
Abstract This chapter deals with the use of spectroscopic methods to monitor conformational changes in membrane transporters and kinetic approaches to time-resolve these changes. It outlines the use of fluorescence spectroscopy to monitor ligand-membrane protein interactions and the use of stopped-flow fluorescence spectroscopy to determine mechanisms of ligand binding and translocation. It also outlines the use of rapid-quench techniques for measuring the rate constants governing the translocation of the substrate across the membrane. To a large extent, these studies are illustrated by reference to the kinetics of substrate and inhibitor binding and translocation by various transporters studied in the author’s laboratory.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
