Abstract
This work is motivated by the need to do quantitative spectrophotometric absorption measurements using small optical fiber needle probes. It has also some relevance with imaging reflectance spectrophotometry of turbid media at high spatial resolution. We have performed experiments to explore the dependence with the scattering and absorption coefficients of the attenuation of the light backscattered through turbid media (liquid samples of absorbing dye and scattering latex microspheres) at small (100 to 200 micrometers) source-detector separations, over a wide range of parameters (l*=250-2000μm, μa=labs-1=0.1-1.5mm-1) which are typical of some biological tissues. Our results suggest that when the characteristic attenuation length labs is smaller than the mean free path 1*, the attenuation changes depend little on the changes of the absorption coefficient but are sensitive to changes of 1*. The opposite is true when labs is small in front of 1*.
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