Abstract
Abstract A simple ultraviolet method has been developed for determining serum or plasma cholinesterase activity. The cholinesterase acts on acetyl- or butyrylthiocholine to release thiocholine, which reacts with 2,2'- or 4,4'-dithiodipyridine, showing absorbance at 324 or 343 nm, respectively. The activity can be measured kinetically and also after incubation for 3 to 5 min at 20° to 37°C. A volume of 10 µl of serum or plasma is sufficient for the determination. This method is well suited for clinical investigation as well as experimental studies.
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