Spectrophotometric Method Development for Estimation of Multicomponent Antidiabetic Drugs in Bulk and Solid Dosage Form

Abstract

Abstract: The aim of the present study was the development and validation of a simple, precise and accurate UVspectrophotometric method for the estimation of metformin (MET), voglibose (VOG) and pioglitazone (PIO) in bulk and tablet dosage form using methAanol as solvent. The method was proposed in the present work, the maximum absorbance was shown at 236nm for MET, 256nm for VOG and 268nm for PIO. The concentration range was 5-25, 0.2-1 and 2-10μg/mL with correlation coefficient 0.0998, 0.999 and 0.999 for MET, VOG and PIO respectively. The various parameters, such as linearity, system suitability, accuracy, precision, ruggedness, limit of detection and limit of quantification were studied as per ICH guidelines. Accuracy of the method was verified by performing recovery studies using simultaneous equation method and found to be 100.04-100.48% for MET, 98.70-99.70% for VOG and 98.67-100.27% for PIO indicates good accuracy of the method. Excellent mean recovery studies for precision, repeatability, ruggedness and sensitivity results showed that the method has been validated successfully, the results are also in accordance with the % RSD values obtained within specified limits. The proposed method was applied to the determination of MET, VOG and PIO, the mean % amount was found to be 100.21 (MET), 98.70 (VOG) & 101.28 (PIO) with % RSD values NMT 2.0% indicates the developed method was successfully applied for analysis of marketed formulation. The developed spectrophotometric method can be employed for routine analysis of MET, VOG and PIO in bulk and tablet formulation.