Spectrophotometric Estimation of Torsemide in Tablet Dosage Form Using Chemical Derivatization Technique.

Abstract

A simple and cost effective spectrophotometric method is described for the determination of Torsemidein pure form and in pharmaceutical formulations. The method is based on the formation derivative when the drug reacts with picric acid solution. The colored derivative solution has an absorption maximum at 350 nm; also obeys Beer’s law in the concentration range 5-25μg /mL. The absorbance was found to increase linearly with increasing concentration of torsemide, which is corroborated by the calculated correlation coefficient value of 0.997. The slope and intercept of the equation of the regression line are 0.035 and 0.002 respectively. The optimum experimental parameters for the reaction have been studied. The validity of the described procedure was assessed. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of Torsemide in pharmaceutical formulations.